| 1 | Mon Nov 7 | vs IU Indy | W88-39 | 1-0 | 1.000 | - | | James H. Hilton Coliseum · Ames, IA |
| 2 | Sun Nov 13 | vsNC A&T | W80-43 | 2-0 | 1.000 | - | | James H. Hilton Coliseum · Ames, IA |
| 3 | Sun Nov 20 | vsMilwaukee | W68-53 | 3-0 | 1.000 | - | | James H. Hilton Coliseum · Ames, IA |
| 4 | Thu Nov 24 | atVillanova | W81-79 (OT) | 4-0 | 1.000 | - | ESPN2 | Moda Center · Portland, OR |
| 5 | Fri Nov 25 | vsUNC | W70-65 | 5-0 | 1.000 | - | ESPN | James H. Hilton Coliseum · Ames, IA |
| 6 | Sun Nov 27 | atUConn | L53-71 | 5-1 | .833 | - | ESPN | Moda Center · Portland, OR |
| 7 | Wed Nov 30 | vsNorth Dakota | W63-44 | 6-1 | .857 | #23 | | James H. Hilton Coliseum · Ames, IA |
| 8 | Sun Dec 4 | vsSt John's | W71-60 | 7-1 | .875 | - | ESPN2 | James H. Hilton Coliseum · Ames, IA |
| 9 | Thu Dec 8 | atIowa | L56-75 | 7-2 | .778 | #20 | FS1 | Carver-Hawkeye Arena · Iowa City, IA |
| 10 | Sun Dec 11 | vsMcNeese | W77-40 | 8-2 | .800 | #20 | | James H. Hilton Coliseum · Ames, IA |
| 11 | Sun Dec 18 | vsW Michigan | W73-57 | 9-2 | .818 | - | | James H. Hilton Coliseum · Ames, IA |
| 13 | Sat Dec 31 | vs12Baylor | W77-62 | 10-2 | .833 | - | ESPNU | James H. Hilton Coliseum · Ames, IA |
| 14 | Wed Jan 4 | atOklahoma | W63-60 | 11-2 | .846 | #25 | ESPN2 | Lloyd Noble Center · Norman, OK |
| 15 | Sat Jan 7 | at17TCU | W69-67 | 12-2 | .857 | #25 | ESPNU | Schollmaier Arena · Fort Worth, TX |
| 16 | Tue Jan 10 | vsTexas Tech | W84-50 | 13-2 | .867 | #14 | | James H. Hilton Coliseum · Ames, IA |
| 17 | Sat Jan 14 | at2Kansas | L60-62 | 13-3 | .813 | #14 | | Allen Fieldhouse · Lawrence, KS |
| 18 | Tue Jan 17 | vs7Texas | W78-67 | 14-3 | .824 | #12 | | James H. Hilton Coliseum · Ames, IA |
| 19 | Sat Jan 21 | atOklahoma St | L59-61 | 14-4 | .778 | #12 | | Gallagher-Iba Arena · Stillwater, OK |
| 20 | Tue Jan 24 | vs5Kansas St | W80-76 | 15-4 | .789 | #12 | ESPNU | James H. Hilton Coliseum · Ames, IA |
| 21 | Sat Jan 28 | atMissouri | L61-78 | 15-5 | .750 | - | ESPN2 | Mizzou Arena · Columbia, MO |
| 22 | Mon Jan 30 | atTexas Tech | L77-80 (OT) | 15-6 | .714 | #13 | ESPN2 | United Supermarkets Arena · Lubbock, TX |
| 23 | Sat Feb 4 | vs8Kansas | W68-53 | 16-6 | .727 | #13 | ESPN | James H. Hilton Coliseum · Ames, IA |
| 24 | Wed Feb 8 | atWest Virginia | L71-76 | 16-7 | .696 | #11 | ESPN2 | WVU Coliseum · Morgantown, WV |
| 25 | Sat Feb 11 | vsOklahoma St | L56-64 | 16-8 | .667 | #11 | ESPN2 | James H. Hilton Coliseum · Ames, IA |
| 26 | Wed Feb 15 | vs22TCU | W70-59 | 17-8 | .680 | #19 | ESPNU | James H. Hilton Coliseum · Ames, IA |
| 27 | Sat Feb 18 | at12Kansas St | L55-61 | 17-9 | .654 | #19 | ESPN | Bramlage Coliseum · Manhattan, KS |
| 28 | Tue Feb 21 | at8Texas | L54-72 | 17-10 | .630 | #23 | LHN | Moody Center · Austin, TX |
| 29 | Sat Feb 25 | vsOklahoma | L50-61 | 17-11 | .607 | #23 | ESPNU | James H. Hilton Coliseum · Ames, IA |
| 30 | Mon Feb 27 | vsWest Virginia | L69-72 | 17-12 | .586 | - | ESPN2 | James H. Hilton Coliseum · Ames, IA |
| 31 | Sat Mar 4 | at7Baylor | W73-58 | 18-12 | .600 | - | ESPN2 | Ferrell Center · Waco, TX |
| 32 | Thu Mar 9 | atBaylor | W78-72 | 19-12 | .613 | - | ESPN | T-Mobile Center · Kansas City, MOS |
| 33 | Fri Mar 10 | atKansas | L58-71 | 19-13 | .594 | - | ESPN | T-Mobile Center · Kansas City, MOS |
| 33 | Fri Mar 17 | vs11Pitt | L41-59 | 19-14 | .576 | #6 | truTV | Greensboro Coliseum · Greensboro, NC |
