| 1 | Mon Nov 4 | vs Miss Valley St | W83-44 | 1-0 | 1.000 | #5 | ESPN+ | James H. Hilton Coliseum · Ames, IA |
| 2 | Mon Nov 11 | vs Kansas City | W82-56 | 2-0 | 1.000 | #7 | ESPN+ | James H. Hilton Coliseum · Ames, IA |
| 3 | Mon Nov 18 | vs IU Indy | W87-52 | 3-0 | 1.000 | #5 | ESPN+ | James H. Hilton Coliseum · Ames, IA |
| 4 | Mon Nov 25 | vs #4Auburn | L81-83 | 3-1 | .750 | #5 | ESPNU | James H. Hilton Coliseum · Ames, IA |
| 5 | Tue Nov 26 | at Dayton | W89-84 | 4-1 | .800 | #5 | ESPNU | UD Arena · Dayton, OH |
| 6 | Wed Nov 27 | vs Colorado | W99-71 | 5-1 | .833 | #5 | ESPN2 | James H. Hilton Coliseum · Ames, IA |
| 7 | Wed Dec 4 | vs #5Marquette | W81-70 | 6-1 | .857 | #6 | ESPN+ | James H. Hilton Coliseum · Ames, IA |
| 8 | Sun Dec 8 | vs Jackson St | W100-58 | 7-1 | .875 | #6 | ESPN+ | James H. Hilton Coliseum · Ames, IA |
| 9 | Thu Dec 12 | at Iowa | W89-80 | 8-1 | .889 | #3 | FS1 | Carver-Hawkeye Arena · Iowa City, IA |
| 10 | Sun Dec 15 | vs Omaha | W83-51 | 9-1 | .900 | #3 | CBS | James H. Hilton Coliseum · Ames, IA |
| 11 | Sun Dec 22 | vs Morgan St | W99-72 | 10-1 | .909 | #3 | ESPN+ | James H. Hilton Coliseum · Ames, IA |
| 12 | Mon Dec 30 | atColorado | W79-69 | 11-1 | .917 | #3 | CBS | CU Events Center · Boulder, CO |
| 13 | Sat Jan 4 | vs #25Baylor | W74-55 | 12-1 | .923 | #3 | CBS | James H. Hilton Coliseum · Ames, IA |
| 14 | Tue Jan 7 | vs Utah | W82-59 | 13-1 | .929 | #3 | ESPN+ | James H. Hilton Coliseum · Ames, IA |
| 15 | Sat Jan 11 | at Texas Tech | W85-84 (OT) | 14-1 | .933 | #3 | ESPN | United Supermarkets Arena · Lubbock, TX |
| 16 | Wed Jan 15 | vs #9Kansas | W74-57 | 15-1 | .938 | #2 | ESPN2 | James H. Hilton Coliseum · Ames, IA |
| 17 | Sat Jan 18 | at West Virginia | L57-64 | 15-2 | .882 | #2 | ESPN+ | WVU Coliseum · Morgantown, WV |
| 18 | Tue Jan 21 | vs UCF | W108-83 | 16-2 | .889 | #3 | ESPN+ | James H. Hilton Coliseum · Ames, IA |
| 19 | Sat Jan 25 | at Arizona St | W76-61 | 17-2 | .895 | #3 | ESPN+ | Desert Financial Arena · Tempe, AZ |
| 20 | Mon Jan 27 | at Arizona | L75-86 (OT) | 17-3 | .850 | #3 | ESPN | McKale Center · Tucson, AZ |
| 21 | Sat Feb 1 | vs Kansas St | L61-80 | 17-4 | .810 | #3 | ESPN2 | James H. Hilton Coliseum · Ames, IA |
| 22 | Mon Feb 3 | at#16Kansas | L52-69 | 17-5 | .773 | #8 | ESPN | Allen Fieldhouse · Lawrence, KS |
| 23 | Sat Feb 8 | vs TCU | W82-52 | 18-5 | .783 | #8 | ESPN+ | James H. Hilton Coliseum · Ames, IA |
| 24 | Tue Feb 11 | atUCF | W77-65 | 19-5 | .792 | #10 | ESPN+ | Addition Financial Arena · Orlando, FL |
| 25 | Sat Feb 15 | vs Cincinnati | W81-70 | 20-5 | .800 | #10 | ESPN2 | James H. Hilton Coliseum · Ames, IA |
| 26 | Tue Feb 18 | vsColorado | W79-65 | 21-5 | .808 | #8 | ESPN+ | James H. Hilton Coliseum · Ames, IA |
| 27 | Sat Feb 22 | at #5Houston | L59-68 | 21-6 | .778 | #8 | ESPN | Fertitta Center · Houston, TX |
| 28 | Tue Feb 25 | at Oklahoma St | L68-74 | 21-7 | .750 | #9 | ESPN+ | Gallagher-Iba Arena · Stillwater, OK |
| 29 | Sat Mar 1 | vs#22Arizona | W84-67 | 22-7 | .759 | #9 | ESPN | James H. Hilton Coliseum · Ames, IA |
| 30 | Tue Mar 4 | vs #23BYU | L85-88 (2OT) | 22-8 | .733 | #10 | ESPN2 | James H. Hilton Coliseum · Ames, IA |
| 31 | Sat Mar 8 | atKansas St | W73-57 | 23-8 | .742 | #10 | CBS | Bramlage Coliseum · Manhattan, KS |
| 32 | Wed Mar 12 | vsCincinnati | W76-56 | 24-8 | .750 | #12 | ESPN | James H. Hilton Coliseum · Ames, IA |
| 33 | Thu Mar 13 | at#17BYU | L92-96 | 24-9 | .727 | #12 | ESPN2 | Marriott Center · Provo, UT |
| 34 | Fri Mar 21 | vs #14Lipscomb | W82-55 | 25-9 | .735 | #3 | TNT | James H. Hilton Coliseum · Ames, IA |
| 35 | Sun Mar 23 | vs #6Ole Miss | L78-91 | 25-10 | .714 | #3 | truTV | James H. Hilton Coliseum · Ames, IA |
