| 1 | Mon Nov 3 | vs Fairleigh | W88-50 | 1-0 | 1.000 | #16 | ESPN+ | James H. Hilton Coliseum · Ames, IA |
| 2 | Thu Nov 6 | vsGrambling | W102-62 | 2-0 | 1.000 | #16 | ESPN+ | James H. Hilton Coliseum · Ames, IA |
| 3 | Mon Nov 10 | vsMississippi St | W96-80 | 3-0 | 1.000 | #16 | ESPNU | James H. Hilton Coliseum · Ames, IA |
| 4 | Mon Nov 17 | vsStonehill | W96-57 | 4-0 | 1.000 | #16 | ESPN+ | James H. Hilton Coliseum · Ames, IA |
| 5 | Mon Nov 24 | at14St John's | W83-82 | 5-0 | 1.000 | #15 | truTV | Carnesecca Arena · Queens, NY |
| 6 | Tue Nov 25 | vsCreighton | W78-60 | 6-0 | 1.000 | #15 | truTV | James H. Hilton Coliseum · Ames, IA |
| 7 | Wed Nov 26 | vsSyracuse | W95-64 | 7-0 | 1.000 | #15 | TNT | James H. Hilton Coliseum · Ames, IA |
| 8 | Wed Dec 3 | vsAlcorn St | W132-68 | 8-0 | 1.000 | #10 | ESPN+ | James H. Hilton Coliseum · Ames, IA |
| 9 | Sat Dec 6 | at1Purdue | W81-58 | 9-0 | 1.000 | #10 | CBS | Mackey Arena · West Lafayette, IN |
| 10 | Thu Dec 11 | vsIowa | W66-62 | 10-0 | 1.000 | #4 | FS1 | James H. Hilton Coliseum · Ames, IA |
| 11 | Sun Dec 14 | vsE Illinois | W78-53 | 11-0 | 1.000 | #4 | ESPN+ | James H. Hilton Coliseum · Ames, IA |
| 12 | Sun Dec 21 | vsLong Beach St | W91-60 | 12-0 | 1.000 | #4 | ESPN+ | James H. Hilton Coliseum · Ames, IA |
| 13 | Mon Dec 29 | vsHou Christian | W89-61 | 13-0 | 1.000 | #3 | ESPN+ | James H. Hilton Coliseum · Ames, IA |
| 14 | Fri Jan 2 | vsWest Virginia | W80-59 | 14-0 | 1.000 | #3 | ESPN2 | James H. Hilton Coliseum · Ames, IA |
| 15 | Wed Jan 7 | atBaylor | W70-60 | 15-0 | 1.000 | #3 | Peacock | Ferrell Center · Waco, TX |
| 16 | Sat Jan 10 | vsOklahoma St | W83-71 | 16-0 | 1.000 | #3 | ESPN2 | James H. Hilton Coliseum · Ames, IA |
| 17 | Tue Jan 13 | atKansas | L63-84 | 16-1 | .941 | #2 | ESPN | Allen Fieldhouse · Lawrence, KS |
| 18 | Sat Jan 17 | atCincinnati | L70-79 | 16-2 | .889 | #2 | Peacock | Fifth Third Arena · Cincinnati, OH |
| 19 | Tue Jan 20 | vsUCF | W87-57 | 17-2 | .895 | #9 | CBSSN | James H. Hilton Coliseum · Ames, IA |
| 20 | Sat Jan 24 | atOklahoma St | W84-71 | 18-2 | .900 | #9 | Peacock | Gallagher-Iba Arena · Stillwater, OK |
| 21 | Thu Jan 29 | vsColorado | W97-67 | 19-2 | .905 | #8 | FS1 | James H. Hilton Coliseum · Ames, IA |
| 22 | Sun Feb 1 | atKansas St | W95-61 | 20-2 | .909 | #8 | FOX | Bramlage Coliseum · Manhattan, KS |
| 23 | Sat Feb 7 | vsBaylor | W72-69 | 21-2 | .913 | #7 | ESPN | James H. Hilton Coliseum · Ames, IA |
| 24 | Tue Feb 10 | atTCU | L55-62 | 21-3 | .875 | #5 | FS1 | Ed and Rae Schollmaier Arena · Fort Worth, TX |
| 25 | Sat Feb 14 | vs9Kansas | W74-56 | 22-3 | .880 | #5 | ABC | James H. Hilton Coliseum · Ames, IA |
| 26 | Mon Feb 16 | vs2Houston | W70-67 | 23-3 | .885 | #6 | ESPN | James H. Hilton Coliseum · Ames, IA |
| 27 | Sat Feb 21 | at23BYU | L69-79 | 23-4 | .852 | #6 | ESPN | Marriott Center · Provo, UT |
| 28 | Tue Feb 24 | atUtah | W75-59 | 24-4 | .857 | #4 | FS1 | Jon M. Huntsman Center · Salt Lake City, UT |
| 29 | Sat Feb 28 | vs16Texas Tech | L73-82 | 24-5 | .828 | #4 | CBS | James H. Hilton Coliseum · Ames, IA |
