| 1 | Tue Nov 9 | vs Kennesaw St | W84-73 | 1-0 | 1.000 | - | | James H. Hilton Coliseum · Ames, IA |
| 2 | Fri Nov 12 | vs Oregon St | W60-50 | 2-0 | 1.000 | - | | James H. Hilton Coliseum · Ames, IA |
| 3 | Tue Nov 16 | vs Alabama St | W68-60 | 3-0 | 1.000 | - | | James H. Hilton Coliseum · Ames, IA |
| 4 | Sun Nov 21 | vs Grambling | W82-47 | 4-0 | 1.000 | - | | James H. Hilton Coliseum · Ames, IA |
| 5 | Wed Nov 24 | vs Xavier | W82-70 | 5-0 | 1.000 | - | ESPNU | James H. Hilton Coliseum · Ames, IA |
| 6 | Fri Nov 26 | at Memphis | W78-59 | 6-0 | 1.000 | - | ESPN2 | FedExForum · Memphis, TN |
| 7 | Wed Dec 1 | vs AR-Pine Bluff | W83-64 | 7-0 | 1.000 | #19 | | James H. Hilton Coliseum · Ames, IA |
| 8 | Sat Dec 4 | at Creighton | W64-58 | 8-0 | 1.000 | - | FS1 | CHI Health Center Omaha · Omaha, NE |
| 9 | Thu Dec 9 | vs Iowa | W73-53 | 9-0 | 1.000 | #17 | ESPN2 | James H. Hilton Coliseum · Ames, IA |
| 10 | Sun Dec 12 | vs Jackson St | W47-37 | 10-0 | 1.000 | #17 | | James H. Hilton Coliseum · Ames, IA |
| 11 | Sun Dec 19 | vs SE Louisiana | W77-54 | 11-0 | 1.000 | #11 | | James H. Hilton Coliseum · Ames, IA |
| 12 | Tue Dec 21 | vs Chicago St | W79-48 | 12-0 | 1.000 | #9 | | James H. Hilton Coliseum · Ames, IA |
| 13 | Sat Jan 1 | vs #1Baylor | L72-77 | 12-1 | .923 | #8 | ESPNU | James H. Hilton Coliseum · Ames, IA |
| 14 | Wed Jan 5 | vs #25Texas Tech | W51-47 | 13-1 | .929 | #11 | ESPNU | James H. Hilton Coliseum · Ames, IA |
| 15 | Sat Jan 8 | at Oklahoma | L66-79 | 13-2 | .867 | #11 | ESPNU | Lloyd Noble Center · Norman, OK |
| 16 | Tue Jan 11 | at #9Kansas | L61-62 | 13-3 | .813 | #15 | | Allen Fieldhouse · Lawrence, KS |
| 17 | Sat Jan 15 | vs #21Texas | W79-70 | 14-3 | .824 | #15 | | James H. Hilton Coliseum · Ames, IA |
| 18 | Tue Jan 18 | at#18Texas Tech | L60-72 | 14-4 | .778 | #15 | ESPNU | United Supermarkets Arena · Lubbock, TX |
| 19 | Sat Jan 22 | vs TCU | L44-59 | 14-5 | .737 | #15 | ESPN2 | James H. Hilton Coliseum · Ames, IA |
| 20 | Wed Jan 26 | at Oklahoma St | W84-81 (OT) | 15-5 | .750 | #23 | | Gallagher-Iba Arena · Stillwater, OK |
| 21 | Sat Jan 29 | vs Missouri | W67-50 | 16-5 | .762 | - | ESPNU | James H. Hilton Coliseum · Ames, IA |
| 22 | Tue Feb 1 | vs#10Kansas | L61-70 | 16-6 | .727 | #20 | ESPN | James H. Hilton Coliseum · Ames, IA |
| 23 | Sat Feb 5 | at#23Texas | L41-63 | 16-7 | .696 | #20 | LHN | Moody Center · Austin, TX |
| 24 | Tue Feb 8 | at West Virginia | L63-79 | 16-8 | .667 | - | | WVU Coliseum · Morgantown, WV |
| 25 | Sat Feb 12 | vs Kansas St | L69-75 (OT) | 16-9 | .640 | - | ESPNU | James H. Hilton Coliseum · Ames, IA |
| 26 | Tue Feb 15 | atTCU | W54-51 | 17-9 | .654 | - | ESPNU | Ed and Rae Schollmaier Arena · Fort Worth, TX |
| 27 | Sat Feb 19 | vsOklahoma | W75-54 | 18-9 | .667 | - | | James H. Hilton Coliseum · Ames, IA |
| 28 | Wed Feb 23 | vsWest Virginia | W84-81 | 19-9 | .679 | - | ESPNU | James H. Hilton Coliseum · Ames, IA |
| 29 | Sat Feb 26 | atKansas St | W74-73 | 20-9 | .690 | - | ESPNU | Bramlage Coliseum · Manhattan, KS |
| 30 | Wed Mar 2 | vsOklahoma St | L36-53 | 20-10 | .667 | - | | James H. Hilton Coliseum · Ames, IA |
| 31 | Sat Mar 5 | at#3Baylor | L68-75 | 20-11 | .645 | - | ESPN2 | Ferrell Center · Waco, TX |
| 32 | Thu Mar 10 | atTexas Tech | L41-72 | 20-12 | .625 | - | ESPN2 | United Supermarkets Arena · Lubbock, TX |
| 33 | Fri Mar 18 | at #6LSU | W59-54 | 21-12 | .636 | #11 | TBS | Pete Maravich Assembly Center · Baton Rouge, LA |
| 34 | Sun Mar 20 | at #3Wisconsin | W54-49 | 22-12 | .647 | #11 | TNT | Kohl Center · Madison, WI |
| 35 | Fri Mar 25 | at #10Miami | L56-70 | 22-13 | .629 | #11 | TBS | Watsco Center · Coral Gables, FL |
