| 1 | Mon Sep 14 | at Giants | W26-16 | 1-0 | 1.000 | MetLife Stadium · East Rutherford, NJ |
| 2 | Sun Sep 20 | vsBroncos | W26-21 | 2-0 | 1.000 | Acrisure Stadium · Pittsburgh, PA |
| 3 | Sun Sep 27 | vsTexans | W28-21 | 3-0 | 1.000 | Acrisure Stadium · Pittsburgh, PA |
| --bye week-- |
| 5 | Sun Oct 11 | vsEagles | W38-29 | 4-0 | 1.000 | Acrisure Stadium · Pittsburgh, PA |
| 6 | Sun Oct 18 | vsBrowns | W38-7 | 5-0 | 1.000 | Acrisure Stadium · Pittsburgh, PA |
| 7 | Sun Oct 25 | atTitans | W27-24 | 6-0 | 1.000 | Nissan Stadium · Nashville, TN |
| 8 | Sun Nov 1 | atRavens | W28-24 | 7-0 | 1.000 | M&T Bank Stadium · Baltimore, MD |
| 9 | Sun Nov 8 | atCowboys | W24-19 | 8-0 | 1.000 | AT&T Stadium · Arlington, TX |
| 10 | Sun Nov 15 | vsBengals | W36-10 | 9-0 | 1.000 | Acrisure Stadium · Pittsburgh, PA |
| 11 | Sun Nov 22 | atJaguars | W27-3 | 10-0 | 1.000 | TIAA Bank Field · Jacksonville, FL |
| 12 | Wed Dec 2 | vsRavens | W19-14 | 11-0 | 1.000 | Acrisure Stadium · Pittsburgh, PA |
| 13 | Mon Dec 7 | vsCommanders | L17-23 | 11-1 | .917 | Acrisure Stadium · Pittsburgh, PA |
| 14 | Sun Dec 13 | atBills | L15-26 | 11-2 | .846 | Highmark Stadium · Orchard Park, NY |
| 15 | Mon Dec 21 | atBengals | L17-27 | 11-3 | .786 | Paycor Stadium · Cincinnati, OH |
| 16 | Sun Dec 27 | vsColts | W28-24 | 12-3 | .800 | Acrisure Stadium · Pittsburgh, PA |
| 17 | Sun Jan 3 | atBrowns | L22-24 | 12-4 | .750 | FirstEnergy Stadium · Cleveland, OH |
| WC | Sun Jan 10 | vsBrowns | L37-48 | 12-4 | .750 | Acrisure Stadium · Pittsburgh, PA |
